PeptiMatrix™ Core

Available in a range of stiffnesses, PeptiMatrix™ Core can also be further customised by the end user with selected matrix components alongside your cells to build fully-defined in vitro environments.

PeptiMatrix™ Core is an innovative, self-assembling peptide hydrogel (SAPH) that was designed to address all the shortcomings of current in vitro model platforms.

It is well-defined, reproducible, optically transparent, and entirely animal-free. It is also supplied ready-to-use, in a range of different stiffnesses, and is stable at room temperature. All you need to do is combine with your cells of interest and plate out. It is also customisable by the end user by encapsulating selected matrix proteins or glycosaminoglycans (GAGs) alongside cells to build more representative models of development and disease.

Validation

Because PeptiMatrix™ Core is available in a range of different stiffnesses, and because it can be further customised by the end user with selected matrix proteins or glycoasminoglycans (GAGs), it can be used to create a variety of tissue mimics and is compatible across a range of different applications.

This technology has been successfully trialled in partner labs across the globe and has been so far validated for the following cell types and applications:

• hiPSCs

• Expansion/maintenance of pluripotency

• Differentiation to various cell lines

• E14TGa mouse embryonic stem cells*

• Expansion/maintenance of pluripotency

• Differentiation to β-III-tubulin+ neural progenitor cells

• Differentiation to GFAP+ glial cells

• Also including various knock-out cells lines

• NIH3T3 mouse embryonic fibroblasts*

• MCF7 breast cancer cells*

• DCIS breast epithelial cells*

• MCF10A breast epithelial cells*

• HMFU19 human mammary fibroblasts*

• Including co-culture with MCF7, DCIS, and MCF10A

• HCT116 colorectal cancer cells*

• Primary breast cancer cells (PDXs)*

• Including expansion and passaging (without animals!)

• Primary acute myeloid leukemia (AML) cells*

• Including drug screening of FDA repurposed drug

• HepG2 human hepatoma cells

validation by PeptiMatrix™ team or by collaborating groups at the University of Nottingham. Other cell lines/applications have been validated by external groups working with our gels.